Edvotek 335 RT-PCR A Model for the Molecular Biology of HIV Replication

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ASIN B004W7QRJ0

Specifications for this item

Brand Name: Edvotek Inc

Mfr Part #: 335 EDVA7

Pkg Qty: 1

Specifications: For 6 sets of reactions

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Key Features

For 6 sets of reactions
Kit includes: instructions, RNA template, primer mix, RT-PCR reaction beads, RNase-Free water, standard DNA markers, agarose, electrophoresis buffer, InstaStain Ethidium Bromide, gel loading solution
You need: thermal cycler, horizontal gel electrophoresis apparatus, power supply, automatic micropipet with tips, balance, microwave oven or hot plate, waterbath
All you need: UV transilluminator, pipet pump or bulb, lab glassware, distilled water
Reverse transcription 35 min, PCR 120 min, gel prep 30 min, electrophoresis 45 min, staining/destaining 2 min

Package Details

Shipping Weight: 0.9 pounds
Package Size: 10.9 x 7.3 x 3.2 inches

Product Description

A specific mRNA is reverse transcribed to double-stranded DNA. This DNA product is then amplified by PCR. This reaction demonstrates the mode of replication of HIV, which contains reverse transcriptase. This experiment is the first introduction of a commercial RNA experiment for the classroom laboratory. Kit includes: instructions, RNA template, primer mix, RT-PCR reaction beads, RNase-Free water, standard DNA markers, agarose, electrophoresis buffer, InstaStain ethidium bromide and gel loading solution. You need: thermal cycler, horizontal gel electrophoresis apparatus, power supply, automatic micropipet with tips, balance, microwave oven or hot plate, waterbath, UV transilluminator, pipet pump or bulb, lab glassware and distilled water.

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