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Package Size: 10.6 x 7.4 x 3.3 inches
In this experiment, students actually purify the restriction enzyme, Eco RI This procedure utilizes an ion exchange chromatography step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by agarose gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for college level courses. Kit includes: instructions, ion exchange matrix, chromatography columns, E.coli cell extract, equilibration and elution buffer, Lambda DNA, Lambda/Eco RI marker, KCl, glycerol, dilution and reaction buffers, gel loading solution, agarose, electrophoresis buffer and stain. You need: horizontal gel electrophoresis apparatus, power supply, UV visualization system, waterbath, microcentrifuge, microwave or hot plate, UV spectrophotometer and cuvettes, automatic micropipet with tips, ring stands and clamps, 10 ml pipets, lab glassware, ice and ice buckets.
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